Peckys, D.B., Gaa, D., Alansary, D., Niemeyer, B.A. & de Jonge, N., Supra-molecular assemblies of ORAI1 at rest precede local accumulation into punctae after activation. Int. J. Mol. Sci., 22, 799, 2021..
Peckys, D.B., Gaa, D., Alansary, D., Niemeyer, B.A. & de Jonge, N., SWeinberg, F., Peckys, D.B. & de Jonge, N., EGFR expression in HER2-driven breast cancer cells. Int. J. Mol. Sci. 21, 9008-1-19, 2020.
Peckys, D.B., Quint, C. & de Jonge, N., Determining the efficiency of single molecule quantum dot labeling of HER2 in breast cancer cells. Nano Lett. 20, 7948–7955, 2020.
Peckys, D.B., Macías-Sánchez, E. & de Jonge, N., Liquid phase electron microscopy of biological specimens. MRS Bulletin, 45, 754-760, 2020.
Alansary, D., Peckys, D.B., Niemeyer, B.A. & de Jonge, N., Detecting single ORAI1 proteins within the plasma membrane reveals higher order channel complexes. J. Cell Sci. 133, jcs240358-1-12, 2020.
Fehling-Kaschek, M., Peckys, D.B., Kaschek, D., Timmer, J., & Niels de Jonge, Mathematical modeling of drug-induced receptor internalization in the HER2-positive SKBR3 breast cancer cell-line, Scientific Reports, 9, 12709, 2019. key paper
Peckys, D.B., Hirsch, D., Gaiser, T., & de Jonge, N., Visualisation of HER2 homodimers in single cells from HER2 overexpressing primary formalin fixed paraffin embedded tumour tissue. Mol Med., 25(1):42, 2019 key paper
Peckys, D.B., de Jonge, N., Hannig M., Oil droplet formation on pellicle covered tooth surfaces studied with environmental scanning electron microscopy environmental scanning electron microscopy. Journal of Microscopy, 274(3): p. 158-167, 2019.
Peckys, D.B.& de Jonge, N., Correlative light- and and electron microscopy of membrane proteins within intact cells in liquid. J. Phys. D: Appl. Phys., D., 51, 443001-1-42, 2018.
Parker, K., Trampert, P., Tinnemann, V., Peckys, D.B., Dahmen T. & de Jonge, N., Linear Chains of HER2 Receptors Found in the Plasma Membrane Using Liquid-Phase Electron Microscopy. Biophys. J., 115, 503-513, 2018.
Vanhecke, D., Kuhn, D.A., Jimenez de Aberasturi, D., Balog, S., Milosevic, A., Urban, D., Peckys, D.B., de Jonge, N., Parak, W.J., Petri-Fink, A. & Rothen-Rutishauser, B., Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages. Beilstein Journal of Nanotechnology, 8, 2396–2409, 2017.
Dahmke, I.N., Verch, A., Hermannsdörfer, J., Peckys, D.B., Weatherup, R., Hofmann, S. & de Jonge, N., Graphene liquid-enclosure for single-molecule analysis of membrane proteins in whole cells using electron microscopy. ACS Nano, 11, 11108-11117, 2017. key paper
Peckys, D.B., Korf, U., Wiemann, S. & de Jonge, N., Liquid-phase electron microscopy of molecular drug response in breast cancer cells reveals irresponsive cell subpopulations related to lack of HER2 homodimers. Molecular Biology of the Cell, 28, 3193-3202, 2017. key paper
Peckys, D.B., Stoerger C., Latta, L., Wissenbach, U., Flockerzi, V. & de Jonge, N., The stoichiometry of the TMEM16A ion channel determined in intact plasma membranes of COS-7 cells using liquid-phase electron microscopy. Journal of Structural Biology, 199, 102-113, 2017.
Peckys, D.B.& de Jonge, N., Live cell electron microscopy is probably impossible. ACS Nano, 10, 9061-9063, 2016.
Peckys, D.B., Alansary, D., Niemeyer, B.A. & de Jonge, N., Visualizing quantum dot labeled ORAI1 proteins in intact cells via correlative light- and electron microscopy. Microscopy and Microanalysis,22, 902-912, 2016.
Hermannsdörfer, J., Tinnemann, V., Peckys, D.B.& de Jonge, N., The effect of electron beam irradiation in environmental scanning transmission electron microscopy of whole cells in liquid. Microscopy and Microanalysis, 22, 656-665, 2016.
Peckys, D.B.and de Jonge, N., Studying the stoichiometry of epidermal growth factor receptor on intact cells using correlative microscopy. Journal of Visualized Experiments, 103:e53186,2015.
Peckys, D.B., Korf, U. and de Jonge, N., Local variations of HER2 dimerization in breast cancer cells discovered by correlative fluorescence- and liquid electron microscopy. Science Advances, 1:e1500165, 2015. key paper
Peckys, D.B.and de Jonge, N., Liquid scanning transmission electron microscopy: imaging protein complexes in their native environment in whole eukaryotic cells. Microscopy and Microanalysis, 20, 346-365. 2014.
Peckys, D.B.and de Jonge, N., Gold Nanoparticle Uptake in Whole Cells in Liquid Examined by Environmental Scanning Electron Microscopy, Microscopy and Microanalysis, 20, 189-198, 2014.
Peckys, D.B., Baudoin, J.P., Eder, M., Werner, U. and de Jonge, N., Epidermal growth factor receptor subunit locations determined inhydrated cells with environmental scanning electron microscopy. Scientific Reports,3:e2626, 2013.
Ring, E.A., Peckys, D.B., Dukes, M.J., Baudoin, J.P. and de Jonge, N., Silicon nitride windows for electron microscopy of whole cells. Journal of Microscopy, 243(3): p. 273-283, 2011.
Peckys, D.B., Mazur, P., Gould, K.L. and de Jonge, N., Fully Hydrated Yeast Cells Imaged with Electron Microscopy. Biophysical Journal, 100(10): 2522-2529, 2011. key paper
Peckys, D.B.and de Jonge, N., Visualizing Gold Nanoparticle Uptake in Live Cells with Liquid Scanning Transmission Electron Microscopy. Nano Letters, 11(4): p. 1733-1738, 2011. key paper
Dukes, M.J., Peckys, D.B.and de Jonge, N., Correlative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum-Dot-Labeled Proteins in Whole Cells in Liquid. ACS Nano, 4(7): p. 4110-4116, 2010.
de Jonge, N., Poirier-Demers, N., Demers, H., Peckys, D.B.and Drouin, D., Nanometer-resolution electron microscopy through micrometers-thick water layers. Ultramicroscopy, 110(9): p. 1114-1119, 2010.
Peckys, D.B., Veith, G.M., Joy, D.C. and de Jonge, N., Nanoscale Imaging of Whole Cells Using a Liquid Enclosure and a Scanning Transmission Electron Microscope. Plos One, 4(12):e8214, 2009.
de Jonge, N., Peckys, D.B., Kremers, G.J. and Piston, D.W., Electron microscopy of whole cells in liquid with nanometer resolution. Proceedings of the National Academy of Sciences of the United States of America, 106(7): p. 2159-2164, 2009. key paper
Book Chapters
Peckys, D.B.& de Jonge, N., Liquid scanning transmission electron microscopy for studying biological function in whole cells. in: Liquid Cell Electron Microscopy ed. Ross, F.M., Cambridge University Press, 2016.
de Jonge, N., Pfaff, M. and Peckys, D.B., Practical aspects of electron microscopy in liquid. Advances in Imaging and Electron Physics, 186, 1-37, 2014.
Peckys, D.B., Bandmann, V. and de Jonge, N., Correlative fluorescence- and scanning transmission electron microscopy of quantum dot labeled proteins on whole cells in liquid. Correlative Light and Electron Microscopy II, Methods in Cell Biology, 124, 305-322, 2014.
Peckys, D.B., Dukes, M.J. and de Jonge, N., Correlative fluorescence- and electron microscopy of quantum dot labeled proteins on whole cells in liquid. Electron Microscopy: Methods and Protocols, 527-540, 2014.
Peckys, D., Eder, M., Werner, U. and de Jonge, N., Visualization of EGF Receptor Dimers on Wet and Intact Eukaryotic Cells in an Environmental Scanning Electron Microscope. Advances in Imaging and Electron Physics, 179, 155-157, 2013.
de Jonge, N. and Peckys, D., Scanning Transmission Electron Microscopy (STEM) of Whole Eukaryotic Cells in Liquid. Advances in Imaging and Electron Physics, 179, 189-191, 2013.
de Jonge, N., Sougrat R., Peckys, D.B., Lupini, A.R. and Pennycook, S.J., 3-dimensional aberration corrected scanning transmission electron microscopy for biology, in Nanotechnology in Biology and Medicine, 13.1-13.27, 2007.
Invited Lectures
Peckys, D.B., Alansary, D., Niemeyer, B.A. & de Jonge, N., ASingle protein visualization in whole cells: an introduction to sample preparation and imaging by liquid-phase electron microscopy.Microscopy Conference 2019, Berlin, Germany, 1-5 September 2019.
Peckys, D.B., Dahmke, I.N., Alansary, D., Niemeyer, B.A. & de Jonge, N., Application of Liquid Phase Scanning Transmission Electron Microscopy Methods for the Quantitative Study of Membrane Proteins in Whole Cells.Microscopy & Microanalysis 2018, Baltimore, USA, 5-9 August 2018.
Peckys, D.B., Dahmke, I.N., Alansary, D., Niemeyer, B.A. & de Jonge, N., Liquid-phase electron microscopy for studying membrane proteins and nanoparticles in intact cells.Microscopy characterisation of organic-inorganic interfaces, RMS, London, UK. 22-23 February, 2018.
Peckys, D. B., Korf, U., Wiemann, S. & de Jonge, N., Role of heterogeneity in cancer cells examined through quantitative analysis of single HER2 protein distribution and activation status, CISCEM 2016 – 3rd Conference on In-Situ and Correlative Electron Microscopy, Saarbrücken, Germany, October 11-12, 2016.
Peckys, D.B., New visions into membrane proteins with environmental scanning electron microscopy in liquid, Seminars of SFB 894 Ca2+signals: Molecular Mechanisms and Integrative Functions, Homburg, Germany, September 22, 2016.
Peckys, D.B., Examining the heterogeneity of growth factor receptor complex formation in intact breast cancer cells in liquid state with correlative light- and electron microscopy. Microscopy Congress 2015, London, UK, November 30–December 1, 2015.
Peckys, D.B.and de Jonge, N., Studying protein distribution and complex formation in whole, hydrated eukaryotic cells with environmental scanning electron microscopy. Workshop SFB 1027 Physical modeling of non-equilibrium processes in biological systems, Homburg, Germany, December 12, 2014.
Peckys, D.B. and de Jonge, N., Environmental scanning electron microscopy for quantitative studies of the fate of gold nanoparticles in intact and hydrated cells. CMD 25 – JMC 14 Condensed Matter, Paris, France, August 24-29, 2014.
Peckys, D.B.and de Jonge, N., Scanning transmission electron microscopy of gold nanoparticles in whole eukaryotic cells in water, 13th meeting of the French microscopy society, Nantes, France, July 02-05, 2013.Peckys, D.B., Scanning transmission electron microscopy of nanoparticles in whole eukaryotic cells in water. Helmholtz-Institut für Pharmazeutische Forschung Saarland, Saarbrücken, Germany, November 13, 2012.